Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The class A scavenger receptor MARCO is the major phagocytic receptor for Clostridium sordellii expressed by human decidual macrophages

doi: 10.4049/jimmunol.1000989

Figure Lengend Snippet: A, Human DMs were pretreated with sMARCO (100 μg/ml) or the SR-AI/II-binding agent acetylated low density lipoprotein (AcLDL; 100 μg/ml) for 30 min and the phagocytosis of FITCC. sordellii was quantified by fluorometry after 180 min as detailed in Materials and Methods. B, Human DMs were pretreated with mouse anti-human monoclonal IgG directed against CD36 or CD163 (or an isotype control) at 50 μg/ml for 30 min and the phagocytosis of FITCC. sordellii was quantified by fluorometry after 180 min as detailed in Materials and Methods. Fucoidan pretreatment for 30 min at 500 μg/ml used as positive control for each. Data are representative from three independent experiments performed in octuplet with similar results. Results are expressed as a percentage of relative to untreated cells. **, P < 0.01 and ***, P < 0.001 vs. untreated cells.

Article Snippet: Recombinant mouse soluble MARCO (sMARCO) and monoclonal anti-human a monoCD163 (clone 215927) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Binding Assay, Control, Positive Control